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* [https://github.com/FloraVincent/DIDB Diatom Interaction Database (DIDB)] (last updated 2019)
* [https://github.com/FloraVincent/DIDB Diatom Interaction Database (DIDB)] (last updated 2019)


Description of the [[Project:Curation workflow|curation workflow]]
Documentation of the workflow and project administration is linked from [[Project:Main]].


==Explore the data==
==Explore the data==

Revision as of 07:54, 23 April 2024

Introduction

This project aims to describe symbiotic interactions between protists and prokaryotes as Linked Open Data. Information in the database is compiled from the scientific literature; over 500 symbiotic interactions described in 300 scientific publications are represented at present. The database is hosted on Wikibase Cloud, a hosting service for Wikibase instances provided by Wikimedia Deutschland.

What can the database be used for?

  • Search and browse symbiotic interactions by biological taxonomy, leveraging cross-references to external taxonomies and databases
  • Find interactions that were described in earlier literature but not yet studied with modern methods
  • Programatically find sequence data, literature, etc. by querying the NCBI databases using linked NCBI taxon IDs
  • Share data with GloBI through periodic data exports

What information do we wish to capture about symbiotic interactions?

  • Taxonomy of hosts and symbionts, with links to external databases (primarily NCBI)
  • Localization of symbionts in cellular compartments of the host cell, using Gene Ontology terms
  • Nature of biotic interactions, if this is known, using Relation Ontology terms (although there are some limitations in this ontology for describing mutualistic interactions)
  • Analytical methods used to study the symbioses

This project originated as part of my doctoral dissertation (2017).

Similar projects elsewhere:

Documentation of the workflow and project administration is linked from Project:Main.

Explore the data

Some example entries to see how the data are modeled:

Each interaction statement is supported by one or more references to the scientific literature, linked by their DOI.

Use the Query Service (link on menu bar) to launch SPARQL queries; try the example queries to get started.

Q & A

Why use a single 'interacts with' statement, with qualifiers for interaction type, instead of different properties for each interaction type?
Nature of an interaction is often not fully understood, or may have multiple facets. Coding interaction types as qualifiers allows us to stack multiple functional roles on a single interaction
What is a placeholder taxon?
We would like to model taxonomic relationships ("find taxa that are members of Ciliophora") and also link out to external databases, particularly NCBI. However, there is often a discrepancy between NCBI Taxonomy and the "actual" taxonomy.
For example, the brown ciliate is reported as a Parduczia sp. based on sequence analysis, but the sequences from that study are published under an environmental "ciliate metagenome" identifier on NCBI that is used for convenience to organize records comprising sequences from multiple taxa, rather than a legitimate taxon in the biological sense.
Therefore, the item "brown ciliate" is described here as a placeholder taxon, because it does not have an exact equivalent in the NCBI Taxonomy, although we think it represents a legitimate taxon, based on the information in the cited references.
For Bacteroidales sp. Cc3-010 ectosymbiont of Caduceia versatilis, an SSU rRNA sequence has been published, but it is currently placed in a provisional "taxon" in the NCBI Taxonomy that is again a convenience label for records where taxonomic information is lacking. The property P28 is used to link our placeholder taxon to a representative sequence from the cited reference, for disambiguation should the NCBI Taxonomy be updated in the future.
Why do some interacts with statements link to "unknown value"?
If a symbiont is reported only on the basis of microscopy, without any information on its phylogenetic affiliation, "unknown value" is used for the object of the statement.
If some information is known about its likely taxonomy, e.g. through use of group-specific FISH probes, then a placeholder taxon is created with a temporary name.
The entry Metopus contortus has both an "unknown value" statement and a placeholder symbiont taxon Q442.
Why host this on Wikibase?
This database has seen a number of iterations: starting as a table in a word processor file, to spreadsheets, a custom SQLite database, and an attempt to homebrew a structured data base with XML files and Python scripts. After getting some experience on Wikidata, I found that Wikibase offers the key features that I wanted: flexible and extensible schemata, graphical frontend for manual data entry, options for programmatic data import from tables, integration with external databases, and a sophisticated query interface.
What is the beautiful organism depicted in the logo?
Kentrophoros sp. H
(The logo may not be visible in the mobile version of this site.)
Which types of interactions are in the scope of this database?
Interactions between protists (microbial eukaryotes) and prokaryotes (bacteria and archaea). In a few cases, interactions between two eukaryotic partners, or interactions with viruses, may also be included if they occur in organisms that also participate in protist-prokaryote interactions.
Fungi and macroalgae are generally not included, as are microbiome studies. Reports of putative symbionts described solely by morphology without further phylogenetic characterization may be included if the host organism is somehow notable, or if they are in older literature about host taxa that have not yet been studied with modern methods.